5
Techniques
200+
Structures Solved
1.0 Å
Best Resolution
1–3
Months End-to-End
100%
PDB Deposition
What We Offer
Comprehensive 3D Structure Determination Across Five Platforms
Three-dimensional structure determination lies at the heart of modern drug discovery, mechanistic biology, and protein engineering. Understanding how a protein folds, where substrates and inhibitors bind, and how multi-protein complexes assemble provides the molecular basis for rational therapeutic design.
ICDMO's structural biology platform spans the complete toolkit — from atomic-resolution X-ray crystallography and Cryo-EM through solution-state NMR, rapid SAXS characterization, and fully integrated structure-based drug design. All techniques are delivered under one roof, enabling seamless integrative structural projects.
Suitable For
Drug targets requiring atomic-resolution binding-site characterization
Membrane proteins, GPCRs, and ion channels (Cryo-EM)
Proteins < 50 kDa with dynamic or disordered regions (NMR)
Multi-subunit complexes and assemblies > 200 kDa (Cryo-EM)
Rapid solution-state shape and oligomeric state profiling (SAXS)
Fragment screening and iterative lead optimization campaigns (SBDD)
Mechanism of action studies: substrate, TS, and product complexes
Service Workflow
From Protein to PDB Deposition
01
01
Protein Production
Gene construct design & codon optimization for expression host
E. coli periplasmic, baculovirus/Sf9, or HEK-293 mammalian expression
Affinity (IMAC, Protein A/G) & polishing SEC chromatography
QC panel: SDS-PAGE, DLS, thermal shift assay, SEC-MALS
02
02
Crystallization Screening
High-throughput 96-well sitting-drop & hanging-drop vapor diffusion
2,000+ commercial conditions (Hampton, Molecular Dimensions, Rigaku)
Mosquito and Crystal Gryphon nanoliter dispensing robots
UV CrysCam imaging to distinguish protein vs. salt crystals
03
03
Crystallization Optimization
Fine-tuning pH, precipitant concentration, additives, and temperature
Streak seeding and micro-seed matrix screening (MMS)
Detergent & lipidic cubic phase (LCP) screens for membrane proteins
Co-crystallization and soaking with ligands, inhibitors, or cofactors
04
04
Data Collection & Phasing
Synchrotron radiation beamlines: Diamond, ESRF, APS, SSRF, Spring 8
In-house Cu Kα source for preliminary space-group screening
Cryo-EM: 300 kV FEG instrument, Falcon 4 / K3 direct electron detector
Phasing: MR, SAD/MAD; cryoSPARC / RELION for Cryo-EM SPA
05
05
Structure Determination & Reporting
Iterative model building: Coot, ISOLDE; refinement: PHENIX, REFMAC5, BUSTER
Stringent validation: MolProbity, Rfree, CC*, Ramachandran statistics
PDB deposition with full structure factor / EM map files
Manuscript-quality PyMOL figures and biological interpretation report
Our Techniques
Five Platforms, Full Structural Coverage
Each technique provides unique, complementary information. Our scientists guide you to the right approach for your specific target, timeline, and scientific question.
Applications
Where Structural Biology Makes the Difference
🔬
Drug Target Validation
Confirm druggability; identify binding pockets, allosteric sites and conformational flexibility for rational drug design.
🧩
Fragment-Based Drug Discovery
Crystallographic screening of 500–1,000 fragment libraries to identify novel chemical starting points for your programme.
📈
Lead Optimization
Iterative co-crystal structures guide SAR: visualize binding modes, optimize key interactions and improve selectivity.
⚙️
Mechanism of Action Studies
Capture substrate, transition-state and product complexes to understand catalytic mechanisms and allosteric regulation.
🔧
Protein Engineering
Rational enzyme variant design, antibody humanization, and protein–protein interface engineering guided by atomic structure.
📋
Regulatory & IP Support
High-quality structures for IND applications, biosimilar comparability studies, and patent binding-mode novelty filings.
Case Studies
Representative Projects
Technique Comparison & Timeline
Note: Timelines are estimates for well-behaved samples. Challenging targets may require additional optimization. Contact us for a free feasibility assessment before project initiation.
Free Feasibility Assessment
Share your target details and our structural biologists will recommend the best technique and provide a no-obligation project plan within 24 hours.
Contact Us Online ConsultationStandard Deliverables
✓Atomic coordinate file (PDB format)
✓Structure factor or EM map file
✓Refinement & validation statistics
✓Publication-quality PyMOL figures
✓Biological interpretation report
✓PDB deposition assistance