1,000
Variants / Run (HT)
HPLC ≥95%
Purity Guaranteed
4 Species
Human / Mouse / Rat / Rabbit
≤2 EU/mg
Endotoxin Specification
5 Days
Fastest Turnaround
Service Overview
From Discovery to High-Purity Recombinant Antibody Production
Recombinant antibody technology enables precise sequence-defined production of any antibody format — full-length IgG, Fab, scFv, bispecific, or nanobody — independent of animal immunization timelines. By modifying antibody-encoding genes using recombinant DNA and protein engineering, followed by optimized mammalian cell expression and multi-step chromatographic purification, iCDMO delivers research-grade and therapeutic-grade antibodies with consistent quality across batches.
iCDMO offers two complementary production tracks: a High-Throughput service processing up to 1,000 constructs per run in HEK293 cells for rapid screening, and a Scale-Up service delivering multi-gram quantities with HPLC ≥95% purity and endotoxin ≤2 EU/mg for lead candidate development and in vivo studies.
Suitable For
High-throughput screening of antibody variant libraries (up to 1,000/run)
Recombinant IgG, Fab, scFv, bispecific, and nanobody formats
Human, Mouse, Rat, and Rabbit species IgG subclasses
Therapeutic lead candidate production (HPLC ≥95%, low endotoxin)
In vivo efficacy and PK/PD studies requiring sterile, QC-verified antibody
Biosimilar comparability and formulation development studies
Antibody–drug conjugate (ADC) and ADC payload evaluation
Diagnostic antibody reagent production with batch-to-batch consistency
Service 01
High-Throughput Recombinant Antibody Production
As biological drug development accelerates, demand for high-throughput antibody preparation has grown dramatically. iCDMO's HEK293-based high-throughput platform supports transient expression and magnetic bead purification of hundreds of antibody constructs simultaneously — enabling rapid screening of antibody libraries and variant panels within days.
High-Throughput
Automated purification instruments support simultaneous high-throughput processing. Up to 1,000 constructs produced per run on a single instrument.
Fast Turnaround
Magnetic bead purification system combined with automated instruments enables rapid preparation. Purified antibody delivered within 5 days for 1–100 constructs.
High Quality
All preparations achieve high HPLC purity with guaranteed low endotoxin — suitable for binding assays, SPR, BLI, and cell-based screening without additional clean-up.
Production Workflow
1
Plasmid Preparation
2
Animal Cell Host (HEK293)
3
Transient Transfection
4
Expanded Culture
5
Magnetic Bead Purification
6
QC Screening
Service 02
Scale-Up Recombinant Antibody Preparation
iCDMO has extensive experience in large-scale antibody production and can address the full spectrum of quantity requirements — from milligrams for early characterization to multi-gram batches for in vivo studies and pre-clinical programmes. Antibody expression is performed to the client's specifications with guaranteed yield and quality.
01
01
Gene Synthesis & Vector Construction
Timeline
1–2 weeks
Gene synthesis with codon optimization for HEK293 expression
Antibody sequence cloning into proprietary high-expression mammalian vector
Sequence verification by Sanger sequencing
Plasmid maxiprep preparation to GMP-grade endotoxin specification
Deliverable:Complete gene synthesis with expression vector
02
02
Small-Scale Expression Analysis
Timeline
1–2 weeks
Transient transfection in HEK293 cells at small scale (50–100 mL)
Expression level analysis by ELISA and SDS-PAGE / western blot
Purification by Protein A/G affinity; SEC to assess aggregation
QC confirmation before proceeding to scale-up production
Deliverable:Expression analysis report + electrophoresis data
03
03
Scale-Up Production & Purification
Timeline
1–2 weeks
Flask- to bioreactor-scale production in HEK293 or CHO suspension cells
Primary purification by Protein A affinity chromatography (ÄKTA system)
Endotoxin removal by ion-exchange chromatography (Capto Q / SP)
SEC-HPLC polishing to remove aggregates and achieve monomer >95%
Deliverable:Purified antibody protein (delivered on dry ice)
04
04
Secondary Purification & QC (Optional)
Timeline
2 weeks
Multi-step chromatography: ion exchange + size-exclusion polishing
Closed UV-sterilization system; all operations under sterile conditions
HPLC purity ≥95% guaranteed; endotoxin ≤2 EU/mg (LAL assay)
Final QC package: SDS-PAGE, SEC-HPLC, ELISA activity, endotoxin report
Deliverable:HPLC ≥95% antibody; endotoxin ≤2 EU/mg
Why Choose iCDMO
Service Advantages
High-Throughput Capability
Automated purification instruments and HEK293 magnetic bead systems support simultaneous processing of up to 1,000 antibody variants per run — ideal for library screening and hit validation campaigns.
Multi-Species Expression
Extensive experience expressing Human, Mouse, Rat, and Rabbit IgG subclasses (IgG1, IgG2, IgG4), as well as Fab, scFv, bispecific, and nanobody formats in HEK293 and CHO systems.
HPLC ≥95% Purity Guaranteed
Every scale-up batch undergoes Protein A affinity, ion-exchange, and SEC polishing to deliver HPLC ≥95% purity — meeting the quality bar for therapeutic lead candidate development.
Sterile & Low Endotoxin
Fully closed UV-sterilization production environment. All antibody preparations routinely achieve endotoxin ≤2 EU/mg by LAL assay — suitable for in vivo functional studies and IND-enabling work.
Microgram to Gram Scale
Flexible production scale from microgram quantities for early binding characterization through multi-gram batches for in vivo efficacy studies — all produced under the same QC framework.
Fast 5-Day Delivery
High-throughput recombinant antibody expression supernatant or purified protein can be delivered within 5 business days for batches of 1–100 constructs, supporting rapid iterative screening cycles.
Case Study
Representative Production Results
Full Service Portfolio
All Antibody Discovery & Development Services
Custom Antibody Development
Polyclonal, monoclonal, and recombinant antibody generation against any target antigen — peptide, recombinant protein, cell, or VLP.
Monoclonal Antibody (mAb)
Hybridoma-based and recombinant mAb generation in murine and rabbit systems with high epitope specificity and batch consistency.
Bispecific Antibody
IgG-like bispecific antibody design and production using knob-into-hole, CrossMab, and tandem-scFv formats for dual-target engagement.
VHH / Nanobody Development
AI-assisted camelid-derived VHH nanobody discovery, humanization, and expression — the smallest fully functional antibody format.
Antibody Humanization
CDR grafting, back-mutation optimization, and in silico immunogenicity scoring to convert murine antibodies to therapeutic-grade human frameworks.
Frequently Asked Questions
Note: All antibody production services are for research use unless otherwise stated. GMP-grade production is available upon request with additional regulatory documentation. Contact us to discuss your specific requirements.
Get a Production Quote
Share your antibody sequence, format, and quantity requirements. Our team will respond within 24 hours with a detailed quote and timeline.
Contact Us Online ConsultationStandard QC Package
✓SEC-HPLC purity trace (≥95% monomer)
✓SDS-PAGE (reducing & non-reducing)
✓Endotoxin report by LAL assay
✓Protein concentration (A280 & BCA)
✓ELISA binding activity confirmation
✓Certificate of Analysis (CoA)
✓Stability data on request