100 bp – 200+ kb
99.99% Accuracy
Codon Optimization
5-Day Standard Delivery
100+ Vectors Available
Service Overview
Gene synthesis has become the cornerstone of modern molecular biology, enabling researchers to rapidly obtain any desired DNA sequence without the limitations of natural sources. ICDMO's gene synthesis platform combines high-fidelity DNA assembly with automated error-correction pipelines, and every construct is released against a documented accuracy specification. Whether you require a simple codon-optimized gene, a complex multi-domain protein construct, a synthetic promoter, or a full metabolic pathway cluster, ICDMO provides end-to-end synthesis and cloning services tailored to your research or therapeutic development goals. All synthesized genes undergo rigorous QC including full Sanger sequencing before delivery.
Key Features & Advantages
Ultra-High Accuracy
Error-correction holds sequence accuracy to a ≥99.99% release specification, verified by full Sanger or NGS sequencing before delivery.
Rapid Turnaround
Standard delivery in 5–7 business days for most genes. Express 3-day service available for sequences up to 3 kb. Large gene assemblies quoted individually.
Codon Optimization
AI-driven codon optimization for expression in any host — E. coli, yeast, insect, CHO, HEK293, plant, or custom — boosting protein yields by up to 10-fold.
Comprehensive Vector Menu
100+ pre-validated expression vectors for bacterial, mammalian, insect, and plant expression. Custom vector construction also available.
Complex Gene Assembly
Long gene synthesis up to 200+ kb, multi-fragment assemblies, repeat-rich sequences, high GC content (up to 80%), and synthetic operons all supported.
Full Sequence Verification
Every synthesized gene is verified by complete Sanger sequencing (or NGS for long constructs) before shipment. CoA and sequence trace files included.
Service Scope & Applications
Standard Gene Synthesis
▸Custom gene synthesis: 100 bp to 50 kb, any sequence, any GC content
▸Codon-optimized genes for bacterial, yeast, mammalian, insect, and plant expression
▸Synthetic promoters, UTRs, terminators, and regulatory elements
▸Gene variants: point mutations, truncations, domain swaps, and chimeric constructs
▸Reporter gene constructs: GFP/RFP fusions, luciferase reporters, degron-tagged constructs
Large-Scale & Complex Gene Assembly
▸Long gene synthesis: 50 kb to 200+ kb for chromosomal segments or metabolic clusters
▸Multi-fragment Gibson Assembly and Golden Gate assembly
▸Repeat-rich sequences: telomeres, satellite DNA, VNTR regions
▸GC-rich (up to 80%) and AT-rich sequences with specialized assembly protocols
▸Synthetic operons and gene clusters for metabolic engineering
Cloning Services
▸Subcloning into any vector: restriction-ligation, Gibson Assembly, In-Fusion, TOPO cloning
▸Directional cloning with confirmed reading frame verification
▸Tag addition: His-tag, FLAG, HA, Myc, GST, MBP, SUMO, and custom fusion tags
▸Promoter swapping and reporter gene insertion
▸Sequence-verified, endotoxin-tested plasmid miniprep and maxiprep delivery
Codon Optimization Strategy
ICDMO's codon optimisation workflow considers codon usage bias for the target host organism, mRNA secondary structure stability (minimizing hairpin structures near the start codon), restriction site avoidance for downstream cloning, GC content normalization (40–60% for most hosts), and CpG dinucleotide management for mammalian expression. Reported yield improvements over native sequences vary widely by target and host, so we set expectations per project rather than quoting a single figure.
Quality Control Procedure
1In silico sequence design validation: secondary structure prediction, repeat analysis, restriction site mapping
2Synthesis using high-fidelity polymerase and phosphoramidite chemistry with error-correction enzymatic treatment
3Agarose gel electrophoresis: fragment size confirmation at each assembly step
4Colony PCR screening: positive clone identification and size verification
5Full Sanger sequencing (bidirectional for all constructs <10 kb; NGS for longer constructs)
6Sequence alignment report: every delivered clone confirmed 100% match to ordered sequence
What We Need From You
1The sequence to synthesise, or the protein sequence from which we design the coding sequence
2Expression host and vector, since codon usage and regulatory elements are host-specific
3Required elements: tags, linkers, signal sequences, restriction sites to include or avoid
4Downstream application, including whether the construct will be used for library construction or editing
5Any sequence features that must be preserved exactly, such as regulatory motifs or epitope coding regions
How the Work Is Performed
1Codon optimisation for the target host with codon adaptation index and usage distribution checked
2GC content balancing across windows, avoiding both extremes that impair synthesis and expression
3Removal of repeats, hairpins and secondary structure in the 5-prime region that suppress translation initiation
4Screening for unintended restriction sites, splice signals, polyadenylation signals and cryptic promoters
5mRNA secondary structure prediction across the transcript, with the initiation region assessed specifically
6Synthesis feasibility review flagging regions likely to be difficult or costly to assemble
What You Receive
1Optimised coding sequence ready to order, in annotated FASTA or GenBank format
2Optimisation report comparing the original and optimised sequences on every criterion applied
3Complete vector map where the construct design is part of the scope
4Flagged synthesis-risk regions with alternative designs where a region is problematic
5Documentation of every deliberate sequence change and the reason for it
Interpretation and Limitations
Codon optimisation improves the probability of good expression; it does not guarantee it, and aggressive optimisation can disrupt translational pausing that some proteins require for correct folding. Predicted mRNA structure is an approximation. Where a protein has failed to express previously, sequence optimisation alone may not be the answer, and we say so rather than reissuing a differently optimised sequence. Synthesis and cloning are executed by partner facilities.
Questions We Are Asked Before Scoping
Will codon optimisation increase yield?
▸Usually it helps, sometimes substantially, occasionally not at all. Where a protein is limited by folding, toxicity or degradation rather than translation, optimisation will not fix it, and we assess which case applies before designing.
Can you design the whole construct, not just the coding sequence?
▸Yes — vector choice, regulatory elements, tags, linkers, protease sites and cloning strategy, delivered as an annotated map ready for synthesis.
Do you handle difficult sequences?
▸We flag high-GC regions, long repeats and strong secondary structure during design and propose alternatives, which is considerably cheaper than discovering the problem during assembly.
Service Workflow
1
Sequence Submission & Design Review
Submit your target sequence (FASTA, GenBank, or direct entry). Our bioinformatics team performs in silico analysis for secondary structures, repeats, and potential synthesis challenges. Codon optimization applied if requested.
2
Gene Synthesis & Error Correction
Oligonucleotide pool synthesis followed by PCR-based assembly. Error-correction enzyme treatment removes mismatched bases. Final assembly amplified and purified.
3
Cloning into Target Vector
Synthesized gene cloned into your selected expression vector using restriction-ligation or sequence-independent assembly. Multiple colonies screened by PCR.
4
Sequencing Verification
Positive clones subjected to full Sanger or NGS sequencing. Sequence alignment performed against original order sequence. Only 100% correct clones selected.
5
Plasmid Preparation & QC
Endotoxin-free plasmid preparation (miniprep or maxiprep as specified). OD260/280 purity check. Restriction digest verification. Final QC report generated.
6
Delivery
Lyophilized or in solution (TE buffer or water). Complete documentation: sequence trace files, CoA, restriction map, and cloning map. Shipped with dry ice for international orders.
Service Process
1
Online Consultation
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2
Solution Matching
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3
Service Contract
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4
Project Execution
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5
Data Delivery
Specifications & Turnaround
Note: ICDMO delivers computational (in silico) services. Results prioritise hypotheses for testing; they do not by themselves establish binding, biological activity, efficacy or safety. All deliverables are for research use only and are not intended for diagnostic or therapeutic use. Downstream experimental work is scoped separately and executed through qualified partners.
Get a Custom Quote
Our scientific team responds within 24 hours with a detailed technical proposal and pricing tailored to your project.
Contact Us Online ConsultationStandard Deliverables
✓Sequence-verified final product
✓Full QC documentation
✓Certificate of Analysis (CoA)
✓Electronic data files
✓Project completion report
Why ICDMO?
Computational CDMO Focus
Reproducible, Versioned Pipelines
Confidence & Limitations Reported
Experiment-Aware Recommendations
Response Within One Business Day